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Description de la ressource pédagogique

Description (résumé)

In oviparous animals such as birds, embryonic development occurs in the egg; after oviposition, there is no further possibility of material exchange from the hen to fulfill the physiological needs of the embryo. In such a context, the egg must contain all resources required for survival and proper development of a living organism. During embryonic development, the chorioallantoic membrane (CAM) is a placenta-like structure which is the nexus for many different physiological and metabolic processes including acid-base balance, respiration, and calcium solubilization from the eggshell that is re-allocated for bone and tissue formation. The highly vascularized CAM occupies a strategic position, as it forms a lining under the eggshell and surrounds the embryo from ED12 onwards. The cellular and genetic bases for its protective mechanisms remain to be fully elucidated. One approach to understanding the functions of the CAM is to identify its protein constituents and how they change during development. In this study, we have characterized the CAM proteome at two stages of development (ED12 and ED19), and assessed the embryonic blood serum proteome to determine its contribution. LC/MS/MS-based proteomics allowed the identification of 1470, 1445, and 791 proteins in CAM (ED12), CAM (ED19), and embryonic blood serum (EBS), respectively. In total, 1796 proteins were identified in the entire study. Of these, 175 (ED12), 177 (ED19), and 105 (EBS) were specific to these stages / compartments.

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• Biochimie (572)

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